The Incorporation of N15-glycine by Avian Erythrocytes and Reticulocytes in Vitro

A study of the incorporation of glycine-N(15) by chicken red cells in vitro has shown that: 1. There is no detectable nitrogen turnover in the histone or desoxyribonucleic acid of erythrocytes or reticulocytes. 2. Hemoglobin synthesis in the nucleated reticulocyte proceeds at 2 to 3 times the rate observed in the mature erythrocyte. 3. The uptake of glycine-N(15) by heme is 9 to 14 times the corresponding uptake into hemoglobin, and 12 to 20 times the calculated uptake into globin. 4. Maturation of the red cell results in a decline in the rate of synthesis of both heme and globin, but the deceleration is much more marked in globin. synthesis. 5. No significant differences could be detected in the low N(15) incorporations of nuclear and cytoplasmic hemoglobins.